Enterovirus Quantitative PCR

Rhinovirus

The enteroviruses are well-known etiologic agents of viral meningitis, viral encephalitis, myocarditis, and respiratory tract disease as well as a wide variety of other clinical conditions. Quantitative PCR can be used to detect the presence of the virus as well as track the course of infection.

See below for additional enterovirus virus assay and pathogen-specific information. For online ordering methods click here or contact us.

Assay Sheet

Test ID Code

1400 Enterovirus Real-time qPCR

CPT Code

87799

Clinical Utility

Procedure

Extraction of enterovirus nucleic acid from CSF, followed by reverse transcription of viral RNA, then amplification and detection of cDNA using real-time, quantitative PCR. An internal control is added to ensure the extraction was performed correctly and the PCR reaction was not inhibited.

Specimens

CSF: 1 ml fluid frozen; submitted in a sterile, leakproof tube; ship on dry ice.

Specificity

Detects the entire spectrum of human enteroviruses, including coxsackie A viruses, coxsackie B viruses, echoviruses, polioviruses, and enteroviruses 68 through 71. The assay does not differentiate between serotypes. The primers and probes used in this assay are specific for enteroviruses.

Assay Range

100 copies/ml to 1 x 1010 copies/ml

Turnaround Time

Within 24 hours of receiving specimen

Download Assay Sheet

The CPT codes provided are based on ViraCor’s interpretation of the American Medical Association’s Current Procedural Terminology (CPT) codes and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. ViraCor assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.

PCR tests are performed pursuant to a license agreement with Roche Molecular Systems, Inc.

This assay was developed and the performance characteristics were determined at ViraCor Laboratories. This test is performed in a CLIA certified laboratory. FDA approval is not required for the performance of this test.

Pathogen Overview

Coming soon!

Collection & Shipping

Specimen Source		Collection Procedure	Transport Procedure
Blood	Plasma	2-3 ml separated from whole blood collected in EDTA (lavender top) tube.	Ship at ambient temperature Monday-Friday
	Whole Blood	3-5 ml collected in EDTA (lavender top) tube. Do not freeze.	Ship at ambient temperature Monday-Friday
	ImmuKnow^® Specimens- Whole Blood	2-3 ml collected in a sodium heparin (green top) tube. Maintain temperature by shipping the specimen in 2 inch thick styrofoam with specimen surrounded by ambient temperature gel packs.	Ship ambient for priority overnight delivery Monday‐Friday *Specimen must arrive at ViraCor within 30 hours of collection.*
	Hepatitis Specimens- Whole Blood	7-10 ml in EDTA, ACD Solution A, or PPT sterile tube. Minimum specimen requirement is 2 ml plasma. Separate plasma from cells within 4 hours of collection and freeze. To remove plasma from cells, centrifuge at 1000 xg for 10-15 minutes. Do not clarify by filtration or further centrifugation. If specimen was collected in PPT tube, the entire tube can be frozen if desired following centrifugation.	Ship ambient or frozen Monday-Friday
Body fluid other than blood or urine		Collect 2-3 ml in a sterile screw-cap tube.	Ship at ambient temperature Monday-Friday
Bone Marrow		1-2 ml, collected in an EDTA (lavender top) tube. Do not freeze.	Ship at ambient temperature Monday-Friday
Bronchial Lavage/Bronchial Wash		2-3 ml, collected in sterile screw-cap tube.	Ship at ambient temperature Monday-Friday
CSF		1-1.5 ml in sterile screw-cap tube. Freeze prior to shipment.	Ship on DRY ICE Monday-Friday
Eye swab		Swab the inflamed conjunctiva or corneal lesions. Place swab in 1-2 ml sterile saline or viral transport media in sterile screw-cap tube.	Ship at ambient temperature Monday-Friday
Fecal		Sterile swab (plastic shaft only) or very small (pea size) fecal sample placed in 1-2 ml sterile saline or viral transport in sterile screw-cap tube.	Ship at ambient temperature Monday-Friday
Nasopharyngeal Aspirate/Tracheal Aspirate		2-3 ml collected in sterile saline in sterile screw-cap tube.	Ship at ambient temperature Monday-Friday
Nasopharyngeal Swab		Sterile swab (flexible shaft) placed in 1-2 sterile saline or viral transport media in sterile screw-cap tube. Do not use calcium alginate swab.	Ship at ambient temerpature Monday-Friday
Swab		Sterile swab (plastic shaft only) placed in 1-2 ml sterile saline or viral transport media in sterile screw-cap tube. Do not use calcium alginate swab.	Ship at ambient temperature Monday-Friday
Tissue		Place in a sterile screw-top container, add a small amount of saline to keep moist.	Ship at ambient temperature Monday-Friday Frozen tissue is acceptable
Urine		5 ml sample collected in a sterile urinalysis container. Transfer to a 15 ml sterile screw-cap tube for shipment.	Ship at ambient temperature Monday-Friday
Vesicular Lesion		Collect fluid and cellular material from the base of several fresh vesicles. Place swab in 1-2 mil sterile saline or viral transport media in sterile screw-cap tube. Do not use calcium alginate swab.	Ship at ambient temperature Monday-Friday
Other Specimen		Please inquire.

Shipping

All specimens must be labeled with patient's name and collection date.
A ViraCor Test Request Form must accompany each specimen.
Ship specimens FedEx Priority Overnight to: ViraCor Laboratories | 1001 NW Technology Dr | Lee's Summit MO 64086

PCR tests are performed pursuant to a license agreement with Roche Molecular Systems Inc.
ImmuKnow is a registered trademark of Cylex Incorporated.
Respiratory Viral Panel is a product of Luminex Corporation.

Abstracts & Publications

Coming soon!