Respiratory Viral Panel (RVP) PCR

Respiratory Viral PanelThe Respiratory Viral Panel (RVP) is an FDA cleared for in vitro diagnostic use, PCR-based assay.  RVP is used to detect a broad range of viruses and subtypes representing the majority of circulating respiratory disease-causing pathogens.  Detection of these pathogens will lead to more efficient management of patients with respiratory infections, play a key role in surveillance, and aid in limiting the spread of respiratory viruses through infection control practices.  

The Respiratory Viral Panel was cleared by the FDA for in vitro diagnostic use as a panel only and must be ordered in its entirety. Viral targets include:

Assay Sheet

Test ID

RV00 Respiratory Viral Panel (RVP)

CPT Code

87798   

Clinical Utility

The Respiratory Viral Panel is a comprehensive assay for the detection of a broad range of viruses and subtypes representing the majority of circulating respiratory disease-causing pathogens of particular importance to children, elderly, and immunocompromised patients. Detection of these pathogens will lead to more efficient management of patients with respiratory infections, play a key role in surveillance, and aid in limiting the spread of respiratory viruses through infection control practices.

Procedure

Viral nucleic acid is extracted from the specimen, which undergoes reverse transcription to generate complementary DNA (cDNA). The target cDNA is amplified using polymerase chain reaction (PCR), then analyzed with Luminex® xTag™ technology to detect the presence or absence of each virus in the panel. The Respiratory Viral Panel (RVP) has been cleared by the FDA for in vitro diagnostic use.

Specimens

* Nasopharyngeal swab (NP swab): sterile swab placed in 1-2 ml sterile saline or viral transport media; ship ambient.
**Nasopharyngeal aspirate (NP aspirate), tracheal aspirate, BAL: 2 ml; submitted in a sterile, screw-top tube; ship ambient.

Specificity

Detects 12 Respiratory viral targets: respiratory syncytial virus (RSV) A, respiratory syncytial virus (RSV) B, influenza A, influenza A subtype H1, influenza A subtype H3, influenza B, parainfluenza 1, parainfluenza 2, parainfluenza 3, human metapneumovirus (hMPV), rhinovirus, and adenovirus

Assay Range

Qualitative results (Positive/Not Detected) for: RSV A, RSV B, influenza A, influenza A subtype H1, influenza A subtype H3, influenza B, parainfluenza 1, parainfluenza 2, parainfluenza 3, hMPV, rhinovirus, and adenovirus

Turnaround Time

Within 24 hours of receiving specimen

pdf DOWNLOAD ASSAY SHEET

* NP swab has been cleared by the FDA for use in the RVP assay.
**In-house verification performed to establish as suitable specimen types.

Information derived from Respiratory Viral Panel Package Insert (Luminex Corporation).
Respiratory Viral Panel is a product of Luminex Corporation.
xTAG is a trademark of Luminex Corporation.
Luminex is a registered trademark of Luminex Corporation.
AS10-0108

 

Collection & Shipping

 

 Specimen Source
 Collection Procedure
 Transport Procedure
 Blood
Plasma
2-3 ml separated from whole blood collected in EDTA (lavender top) tube.
 Ship at ambient temperature Monday-Friday
Whole Blood
3-5 ml collected in EDTA (lavender top) tube. Do not freeze.
 Ship at ambient temperature Monday-Friday
 ImmuKnow® Specimens- Whole Blood
2-3 ml collected in a sodium heparin (green top) tube. Maintain temperature by shipping the specimen in 2 inch thick styrofoam with specimen surrounded by ambient temperature gel packs.
 Ship ambient for priority overnight delivery  Monday‐Friday
Specimen must arrive at ViraCor within 30 hours of collection.
 Hepatitis Specimens- Whole Blood
7-10 ml in EDTA, ACD Solution A, or PPT sterile tube. Minimum specimen requirement is 2 ml plasma. Separate plasma from cells within 4 hours of collection and freeze. To remove plasma from cells, centrifuge at 1000 xg for 10-15 minutes. Do not clarify by filtration or further centrifugation. If specimen was collected in PPT tube, the entire tube can be frozen if desired following centrifugation.
 Ship ambient or frozen
Monday-Friday
 Body fluid other than blood or urine
Collect 2-3 ml in a sterile screw-cap tube.
 Ship at ambient temperature Monday-Friday
 Bone Marrow
1-2 ml, collected in an EDTA (lavender top) tube. Do not freeze.
 Ship at ambient temperature Monday-Friday
 Bronchial Lavage/Bronchial Wash
2-3 ml, collected in sterile screw-cap tube.
 Ship at ambient temperature Monday-Friday
 CSF
1-1.5 ml in sterile screw-cap tube. Freeze prior to shipment.
 Ship on DRY ICE
Monday-Friday
 Eye swab
Swab the inflamed conjunctiva or corneal lesions. Place swab in 1-2 ml sterile saline or viral transport media in sterile screw-cap tube.
 Ship at ambient temperature Monday-Friday
 Fecal
Sterile swab (plastic shaft only) or very small (pea size) fecal sample placed in 1-2 ml sterile saline or viral transport in sterile screw-cap tube.
 Ship at ambient temperature Monday-Friday
 Nasopharyngeal Aspirate/Tracheal Aspirate
2-3 ml collected in sterile saline in sterile screw-cap tube.
 Ship at ambient temperature Monday-Friday
 Nasopharyngeal Swab
Sterile swab (flexible shaft) placed in 1-2 sterile saline or viral transport media in sterile screw-cap tube. Do not use calcium alginate swab.
 Ship at ambient temerpature Monday-Friday
 Swab
Sterile swab (plastic shaft only) placed in 1-2 ml sterile saline or viral transport media in sterile screw-cap tube. Do not use calcium alginate swab. 
Ship at ambient temperature Monday-Friday
 Tissue
Place in a sterile screw-top container, add a small amount of saline to keep moist. 
Ship at ambient temperature Monday-Friday Frozen tissue is acceptable 
 Urine
5 ml sample collected in a sterile urinalysis container. Transfer to a 15 ml sterile screw-cap tube for shipment. 
 Ship at ambient temperature Monday-Friday
 Vesicular Lesion
Collect fluid and cellular material from the base of several fresh vesicles. Place swab in 1-2 mil sterile saline or viral transport media in sterile screw-cap tube. Do not use calcium alginate swab.
Ship at ambient temperature Monday-Friday  
 Other Specimen
Please inquire.

Shipping

  • All specimens must be labeled with patient's name and collection date.
  • A ViraCor Test Request Form must accompany each specimen.
  • Ship specimens FedEx Priority Overnight to: ViraCor Laboratories | 1001 NW Technology Dr | Lee's Summit MO 64086

PCR tests are performed pursuant to a license agreement with Roche Molecular Systems Inc.
ImmuKnow is a registered trademark of Cylex Incorporated.
Respiratory Viral Panel is a product of Luminex Corporation.

Abstracts & Publications

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Glanville AR, Scott AI, Morton JM, et al. Intravenous ribavirin is a safe and cost-effective treatment for respiratory syncytial virus infection after lung transplantation. J Heart Lung Transplant. 2005;24(12):2114-2119.

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Ison MG, Hayden F, Kaiser L, Corey L, Boeckh M. Rhinovirus infections in hematopoietic stem cell transplant recipients with pneumonia. Clin Infect Dis. 2003;(36)1139-1143.

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Mahony J, Chong S, Merante F, et al. Development of a respiratory virus panel (RVP) test for detection of twenty human respiratory viruses using multiplex PCR and a fluid microbead-based assay. J Clin Microbiol. 2007;45(9):2965-2970.

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xTAG™ RVP (Respiratory Viral Panel) Package Insert.